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1999), was not clearly validated inthe large clinical trial conducted by the NeonatalResearch Network (Carlo et al. Additionally, it can be used to detectstructural changes, such as large deletions, insertions, duplications and transloca-tions, which account for about 10 % of all reported mutations [ 18].
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If heard as two sounds, the ?rst compo-nent represents mitral valve closure (M1); the second compo-nent represents tricuspid closure (T1).
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